Human AP13 (Apelin 13) ELISA Kit Packaging:96 wells HRP-Streptavidin was added

HRP-Streptavidin was added

The OD value is proportional to the concentration of Human CHEM

And then biotinylated Human OPN5 Antibody is added and binds to OPN5 in the sample

TPPP present in the sample is added and binds to antibodies coated on the wells

Human AP13 (Apelin 13) ELISA Kit Packaging:96 wells HRP-Streptavidin was addedThe Human AP13 (Apelin 13) ELISA Kit measures AP13 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with AP13. AP13 in the sample or standard competes with a fixed amount of biotin labeled AP13 for sites on a pre coated antibody specific to AP13. Free components are washed off. After adding HRP