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Substrate solution is then added and color develops in proportion to the amount of Human ABL2
Then Streptavidin-HRP is added and binds to the Biotinylated PHF7 antibody
The concentration of Rat ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIg
Human RCAN1 (Calcipressin-1) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (RCAN1) Calcipressin 1 ELISA Kit measures Calcipressin 1 in samples. The plate has been pre coated with Human RCAN1 antibody. RCAN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RCAN1 Antibody is added and binds to RCAN1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RCAN1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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