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The absorbance is measured on a microplate reader at 450 nm
only those wells that contain Human ACTN3
Substrate solution is then added and color develops in proportion to the amount of Mouse SPP2
The plate has been pre-coated with Human CEACAM7 antibody
Mouse SYP (Synaptophysin) ELISA Kit SCYB4 The absorbance is measured onThe Mouse (SYP) Synaptophysin ELISA Kit measures Synaptophysin in Mouse samples. The plate has been pre coated with Mouse SYP antibody. SYP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SYP Antibody is added and binds to SYP in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SYP antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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