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PAI1 in the sample or standard competes with a fixed amount of biotin-labeled PAI1 for sites on a pre-coated antibody specific to PAI1
Then a biotinylated detection antibody specific for MIP-3Alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Rat CXCL16
Then Streptavidin-HRP is added and binds to the Biotinylated ADRA2B antibody
Human LAMB4 (Laminin subunit beta-4) ELISA Kit TNFR55 PAI1 in the sample orThe Human LAMB4 (Laminin subunit beta 4) ELISA Kit measures LAMB4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti LAMB4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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