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And then biotinylated Mouse NPHS2 Antibody is added and binds to NPHS2 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated AVP antibody
The concentration of TTN in the sample was calculated by drawing a standard curve
then it binds with Anti-C3C bound to precoated antibody
Rabbit PRF1 (Perforin 1) ELISA Kit Packaging:48 wells And then biotinylated Mouse NPHS2The Rabbit PRF1 (Perforin 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rabbit PRF1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit PRF1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
