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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GPX4
The concentration of Mouse CFD in the samples is then determined by comparing the OD of the samples to the standard curve
E2-tag peptide GVSSTSSDFRDR conjugated to KLH
The concentration of Human RLBP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Bovine IL-4 (Interleukin 4) ELISA Kit TIMD-1 Standards or samples are addedThe Bovine (IL 4) Interleukin 4 ELISA Kit measures Interleukin 4 in Bovine samples. The plate has been pre coated with Bovine IL4 antibody. IL4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine IL4 Antibody is added and binds to IL4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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