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FH present in the sample is added and binds to antibodies coated on the wells
Alpha-actinin Rabbit Polyclonal Antibody (F298)
The concentration of Rabbit MIP1b in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIM3
Human GAN (Gigaxonin) ELISA Kit Packaging:96 wells FH present in the sampleThe Human (GAN) Gigaxonin ELISA Kit measures Gigaxonin in samples. The plate has been pre coated with Human GAN antibody. GAN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GAN Antibody is added and binds to GAN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GAN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
