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IL-4 in the sample or standard competes with a fixed amount of biotin-labeled IL-4 for sites on a pre-coated antibody specific to IL-4
Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CRP are added to the micro ELISA plate wells
Calculate the concentration of SPINK1/TATI in the sample by plotting standard curve
And then biotinylated Human EBI3 Antibody is added and binds to EBI3 in the sample
Rat ASRGL1 (L-asparaginase) ELISA Kit Packaging:96 wells IL-4 in the sample orThe Rat ASRGL1 (L asparaginase) ELISA Kit measures ASRGL1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ASRGL1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
