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When the sample containing Fractalkine is added to the plate
Universal VD in the sample or standard competes with a fixed amount of Universal VD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VD
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C2
The Rat GAL4 (Galectin 4) ELISA Kit is a Sandwich enzyme immunoassay
Canine OCLN (Occludin) ELISA Kit Glioblastoma-derived T-cell suppressor factor When the sample containing FractalkineThe Canine (OCLN) Occludin ELISA Kit measures Occludin in Canine samples. The plate has been pre coated with Canine OCLN antibody. OCLN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine OCLN Antibody is added and binds to OCLN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated OCLN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
