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Then a biotinylated detection antibody specific for Mouse TNF-Beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Human GLα
Only those wells that contain Human BLC
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ECE1
Human 5HT (5-hydroxytryptamine) ELISA Kit IL6Q Then a biotinylated detection antibodyThe Human (5HT) 5 hydroxytryptamine ELISA Kit measures 5 Hydroxytryptamine in samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added
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