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Substrate solution is then added and color develops in proportion to the amount of Rat LTBP2
Then a biotinylated detection antibody specific for IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Porcine IL-1Alpha
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITGb6
Rat ACCPA (anti-cyclic citrullinated peptide antibody) ELISA Kit YT Substrate solution is then addedThe Rat ACCPA (anti cyclic citrullinated peptide antibody) ELISA Kit measures ACCPA in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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