Human CSTB (Cystatin-B) ELISA Kit YKL-40 IGFBP2 present in the sample

IGFBP2 present in the sample is added and binds to antibodies coated on the wells

Substrate solution is then added and color develops in proportion to the amount of Bovine DEFB1

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD34

then added a HRP-conjugated antibody specific to Mouse fT3 to each microplate well and incubated

Human CSTB (Cystatin-B) ELISA Kit YKL-40 IGFBP2 present in the sampleThe Human (CSTB) Cystatin B ELISA Kit measures Cystatin B in samples. The plate has been pre coated with Human CSTB antibody. CSTB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CSTB Antibody is added and binds to CSTB in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CSTB antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.