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Add standard and properly diluted sample into relevant well respectively
Only those wells that contain Mouse LIF
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FGF21
Then a biotinylated detection antibody specific for Mouse IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Mouse TF (Transferrin) ELISA Kit Packaging:96 wells Add standard and properly dilutedThis Mouse TF (Transferrin) ELISA Kit uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse TF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TF and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated.
