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U2 present in the sample is added and binds to antibodies coated on the wells
Human ACTH in the sample or standard competes with a fixed amount of Human ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human ACTH
The OD value is proportional to the concentration of Human VDR
And then biotinylated Mouse KEAP1 Antibody is added and binds to KEAP1 in the sample
Human OC90 (Otoconin 90) ELISA Kit HRG1 U2 present in the sampleThe Human (OC90) Otoconin 90 ELISA Kit measures Otoconin 90 in samples. The plate has been pre coated with Human OC90 antibody. OC90 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human OC90 Antibody is added and binds to OC90 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated OC90 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
