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Then Streptavidin-HRP is added and binds to the Biotinylated PNP antibody
ASIP present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Human CTLA4 Antibody is added and binds to CTLA4 in the sample
The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve
Canine MYO (Myoglobin) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Canine MYO (Myoglobin) ELISA Kit measures MYO in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MYO antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.