Rat HYP (Hydroxyproline) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added and

Then Streptavidin-HRP is added and binds to the Biotinylated CNTF antibody

The plate has been pre-coated with Mouse GLRX5 antibody

Then a biotinylated detection antibody specific for Mouse UCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPA

Rat HYP (Hydroxyproline) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Rat (HYP) Hydroxyproline ELISA Kit measures hydroxyproline in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and color