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Then a biotinylated detection antibody specific for PAI1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Rat CHRM1 antibody
The concentration of Human PGA in the samples is then determined by comparing the OD of the samples to the standard curve
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 24 nm
Human KRT75 (Keratin 75) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (KRT75) Keratin 75 ELISA Kit measures Keratin 75 in samples. The plate has been pre coated with Human KRT75 antibody. KRT75 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KRT75 Antibody is added and binds to KRT75 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KRT75 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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