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Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of SP in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HMMR
a capture antibody-HSF1-biotin-detection antibody complex was formed
Substrate solution is then added and color develops in proportion to the amount of Human ATP2A1
Human Bcl-2 (B-cell Leukemia/Lymphoma 2) ELISA Kit PSF Compare OD450 value of sampleThe Human Bcl 2 (B cell Leukemia Lymphoma 2) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human Bcl 2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Bcl 2 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each
