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Substrate solution is then added and color develops in proportion to the amount of Human FGF6
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GZMH
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NRP1
Then a biotinylated detection antibody specific for S100A8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human CA5B (Carbonic Anhydrase VB, Mitochondrial) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human CA5B (Carbonic Anhydrase VB, Mitochondrial) ELISA Kit measures CA5B in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CA5B antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
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