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DBN1 present in the sample is added and binds to antibodies coated on the wells
The OD value is proportional to the concentration of Human OPG
Then Streptavidin-HRP is added and binds to the Biotinylated GLB1 antibody
And then biotinylated Rat HMGB1 Antibody is added and binds to HMGB1 in the sample
Human Beta-HCG (beta-Hyperglycosylated Chorionic Gonadotropin) ELISA Kit Packaging:96 wells DBN1 present in the sampleThe Human Beta HCG (beta Hyperglycosylated Chorionic Gonadotropin) ELISA Kit measures Beta HCG in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti hCG H antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound
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