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AGER present in the sample is added and binds to antibodies coated on the wells
CRH in the sample or standard competes with a fixed amount of biotin-labeled CRH for sites on a pre-coated antibody specific to CRH
Substrate solution is then added and color develops in proportion to the amount of Human F13B
You can calculate the concentration of Rat IL-22 in the samples by comparing the OD of the samples to the standard curve
Canine IFN-Beta (Interferon beta) ELISA Kit Packaging:96 wells AGER present in the sampleThe Canine IFN Beta (Interferon beta) ELISA Kit measures IFN Beta in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IFN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
