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HCN2 present in the sample is added and binds to antibodies coated on the wells
The concentration of Human IL34 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse BALP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Bovine HSPB1 Antibody is added and binds to HSPB1 in the sample
Rat HSP60 (Heat Shock Protein 60) ELISA Kit Packaging:96 wells HCN2 present in the sampleThe Rat (HSP60) Heat Shock Protein 60 ELISA Kit measures Heat Shock Protein 60 in Rat samples. The plate has been pre coated with Rat HSPD1 antibody. HSPD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HSPD1 Antibody is added and binds to HSPD1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HSPD1 antibody. After incubation unbound Streptavidin HRP is washed away
