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Human αMSH in the sample or standard competes with a fixed amount of Human αMSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human αMSH
only those wells that contain Human IL28B
Substrate solution is then added and color develops in proportion to the amount of Porcine trypsin
The plate has been pre-coated with Human AUTS2 antibody
Rat FLOT1 (Flotillin-1) ELISA Kit MLN 19 Human αMSH in the sampleThe Rat (FLOT1) Flotillin 1 ELISA Kit measures Flotillin 1 in Rat samples. The plate has been pre coated with Rat FLOT1 antibody. FLOT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat FLOT1 Antibody is added and binds to FLOT1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FLOT1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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