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Then a biotinylated detection antibody specific for Human ADAMTS5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 32 nm
The Rat MYH6 (Myosin Heavy Chain 6
only those wells that contain Human SIGLEC6
Human IKZF1 (DNA-binding Protein Ikaros) ELISA Kit THBS2 Then a biotinylated detection antibodyThe Human (IKZF1) DNA binding Protein Ikaros ELISA Kit measures DNA Binding Protein Ikaros in samples. The plate has been pre coated with Human IKZF1 antibody. IKZF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IKZF1 Antibody is added and binds to IKZF1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IKZF1 antibody. After incubation unbound Streptavidin HRP is