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Then a biotinylated detection antibody specific for Human TARC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
only those wells that contain Human IFN Gamma
Only those wells that contain Mouse F9
The genomic DNA in the supernatant can be bound to the purification column
Avi-Tag Mouse mAb Packaging:50 uL Then a biotinylated detection antibodyThe Avi tag is a biotin acceptor peptide1, GLNDIFEAQKIEWHE. The 15 residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain.
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