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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG1 are added to the micro ELISA plate wells
And then biotinylated Human FIS-1 Antibody is added and binds to FIS-1 in the sample
it binds with TMEM120B bound to precoated antibody
Then Streptavidin-HRP is added and binds to the Biotinylated EEF2 antibody
Mouse RasGAP (Ras GTPase Activating Protein) ELISA Kit RSTN Samples (or Standards) and HorseradishThe Mouse RasGAP (Ras GTPase Activating Protein) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse RasGAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RasGAP and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to
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