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IL-2 present in the sample is added and binds to antibodies coated on the wells
The concentration of Human CMA1 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human dLL3
CTHRC1 present in the sample is added and binds to antibodies coated on the wells
Mouse RSPO1 (R-spondin-1) ELISA Kit Packaging:96 wells IL-2 present in the sampleThe Mouse (RSPO1) R spondin 1 ELISA Kit measures R Spondin 1 in Mouse samples. The plate has been pre coated with Mouse RSPO1 antibody. RSPO1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse RSPO1 Antibody is added and binds to RSPO1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RSPO1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
