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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HLA-B27
Then a biotinylated detection antibody specific for Mouse FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human MYH7 in the samples is then determined by comparing the OD of the samples to the standard curve
only those wells that contain Human OC and HRP-conjugated antibody will exhibit a change in color
Human CAPN1 (Calpain-1 Catalytic Subunit) ELISA Kit p55-R Standards or samples are addedThe Human (CAPN1) Calpain 1 Catalytic Subunit ELISA Kit measures Calpain 1 Catalytic Subunit in samples. The plate has been pre coated with Human CAPN1 antibody. CAPN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CAPN1 Antibody is added and binds to CAPN1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CAPN1 antibody. After incubation unbound Streptavidin HRP is
