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The concentration of HDLC in the sample was calculated by drawing a standard curve
it binds with RGS4 bound to precoated antibody
Then Streptavidin-HRP is added and binds to the Biotinylated TNNI3 antibody
a capture antibody-INS-HRP-detection antibody complex was formed
Human F8 (Coagulation Factor Ⅷ) ELISA Kit PYPAF1 The concentration of HDLC inThe Human F8 (Coagulation Factor ) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human F8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F8 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
