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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACTa2
Only those wells that contain CHI3L1
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken LEP
Then a biotinylated detection antibody specific for Mouse C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human CD47 (Leukocyte Surface Antigen Cd47) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Human (CD47) Leukocyte Surface Antigen Cd47 ELISA Kit measures Leukocyte Surface Antigen Cd47 in samples. The plate has been pre coated with Human CD47 antibody. CD47 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD47 Antibody is added and binds to CD47 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CD47 antibody. After incubation unbound Streptavidin HRP is
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