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The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine IL-2
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish TNFa
The concentration of Mouse DUSP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated CD7 antibody
Human HCRT (Orexin) ELISA Kit 8-iso-PGF2a The micro ELISA plate providedThe Human (HCRT) Orexin ELISA Kit measures Orexin in samples. The plate has been pre coated with Human HCRT antibody. HCRT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HCRT Antibody is added and binds to HCRT in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HCRT antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
