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Then Streptavidin-HRP is added and binds to the Biotinylated MMP16 antibody
The assay plate has been pre-coated with mouse anti-Human VSIG4 monoclonal antibody
Substrate solution is then added and color develops in proportion to the amount of Human SEMA3B
NLRP2 present in the sample is added and binds to antibodies coated on the wells
Mouse SPARC/ON (Osteonectin) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Mouse SPARC ON (Osteonectin) ELISA Kit measures SPARC ON in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SPARC ON antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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