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The concentration of Human ERFE in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT14
Then a biotinylated detection antibody specific for IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human FGF2 in the samples is then determined by comparing the OD of the samples to the standard curve
Bovine GH1 (Somatotropin) ELISA Kit Packaging:96 wells The concentration of Human ERFEThe Bovine (GH1) Somatotropin ELISA Kit measures Somatotropin in Bovine samples. The plate has been pre coated with Bovine GH1 antibody. GH1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine GH1 Antibody is added and binds to GH1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GH1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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