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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IL6
Then a biotinylated detection antibody specific for Human OCLN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
only those wells that contain Rat MEPE
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CTNNb1
Mouse CCL27 (C-C Motif Chemokine 27) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Mouse (CCL27) C C Motif Chemokine 27 ELISA Kit measures C C motif chemokine 27 in Mouse samples. The plate has been pre coated with Mouse CCL27 antibody. CCL27 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CCL27 Antibody is added and binds to CCL27 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CCL27 antibody. After incubation unbound Streptavidin HRP is washed
