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Then a biotinylated detection antibody specific for Rat BAX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Human BAMBI
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1
HSD3B1 present in the sample is added and binds to antibodies coated on the wells
Human SRR (Serine Racemase) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (SRR) Serine Racemase ELISA Kit measures Serine racemase in samples. The plate has been pre coated with Human SRR antibody. SRR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SRR Antibody is added and binds to SRR in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SRR antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
