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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IGFBP7
The concentration of Human PABPC1L in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Human FLRT2 Antibody is added and binds to FLRT2 in the sample
the detection sensitivity can be increased by 10-50 times
Rat GSH (Glutathione) ELISA Kit APO2L Standards or samples are addedThe Rat (GSH) Glutathione ELISA Kit measures Glutathione in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and color
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