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Then a biotinylated detection antibody specific for Human ProGRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Rat SHH in the samples is then determined by comparing the OD of the samples to the standard curve
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL-12p40
The concentration of Human GMCSF in the samples is then determined by comparing the OD of the samples to the standard curve
Mouse PADI4 (Protein-arginine Deiminase Type-4) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Mouse (PADI4) Protein arginine Deiminase Type 4 ELISA Kit measures Protein arginine deiminase type 4 in Mouse samples. The plate has been pre coated with Mouse PADI4 antibody. PADI4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PADI4 Antibody is added and binds to PADI4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PADI4 antibody. After incubation unbound
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