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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ECF
METRNL present in the sample is added and binds to antibodies coated on the wells
Then a biotinylated detection antibody specific for Human TSP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Mouse IL17RA
Rat UCN (Urocortin) ELISA KIT TGFA The microtiter plate provided inThe Rat (UCN) Urocortin ELISA KIT measures Urocortin in Rat samples. The plate has been pre coated with Rat UCN antibody. UCN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat UCN Antibody is added and binds to UCN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated UCN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution
