Collagen I Monoclonal Antibody (6E11) NIL16 After TMB substrate solution is

After TMB substrate solution is added

Then a biotinylated detection antibody specific for Rat VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Then a biotinylated detection antibody specific for Human CALP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

You can calculate the concentration of Human COL3α1 in the samples by comparing the OD of the samples to the standard curve

Collagen I Monoclonal Antibody (6E11) NIL16 After TMB substrate solution isCollagen I Mouse Monoclonal Antibody (6E11). Isotype: IgG1. Species Reactivity: Human, Mouse, Rat. Source: Mouse. Antigen: Synthetic Peptide. Composition and storage: 1 mg mL in PBS, pH 7. 4, containing 0. 02% sodium azide and 50% glycerol. Store at 20C. Stable for one year from the date of shipment.