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You can calculate the concentration of Monkey C5a in the samples by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFb2
Then a biotinylated detection antibody specific for Human THP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
and the color intensity is directly proportional to the amount of Human IL-22BP present
HNF1b (Hepatocyte Nuclear Factor 1 Beta) ELISA Kit (Small Sample) ADAM metallopeptidase domain 10 You can calculate the concentrationThe HNF1b (Hepatocyte Nuclear Factor 1 Beta) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human HNF1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human HNF1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
