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SOX2 present in the sample is added and binds to antibodies coated on the wells
You can calculate the concentration of Rat HIF-1α in the samples by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Rat HPRT1
The Bovine IL-6 ELISA kit is a sandwich enzyme immunoassay
Rat VCAM-1 (Vascular Cell Adhesion Protein 1) ELISA Kit Packaging:96 wells SOX2 present in the sampleThe Rat (VCAM 1) Vascular Cell Adhesion Protein 1 ELISA Kit measures Vascular cell adhesion protein 1 in Rat samples. The plate has been pre coated with Rat VCAM 1 antibody. VCAM 1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat VCAM 1 Antibody is added and binds to VCAM 1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated VCAM 1 antibody. After incubation unbound
