Rat IL-6 (Interleukin-6) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added and

Then Streptavidin-HRP is added and binds to the Biotinylated LUM antibody

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAPN1

a capture antibody-PELO-biotin-detection antibody complex was formed

If the sample contains THBS1/TSP-1

Rat IL-6 (Interleukin-6) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Rat IL 6 (Interleukin 6) ELISA Kit measures IL 6 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.