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Then a biotinylated detection antibody specific for Human FN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Rat IP-10/CXCL10
Only those wells that contain Human SCF
The OD value is proportional to the concentration of Human TARC
Bovine IL-8 ELISA Kit S100-B Then a biotinylated detection antibodyThe Bovine IL 8 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Cattle IL8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Cattle IL8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those
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