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Then a biotinylated detection antibody specific for Human SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human MYH9 in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Human IL2 Antibody is added and binds to IL2 in the sample
The concentration of Human WISP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Human CCL28 (C-C motif chemokine 28) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human CCL28 (C C motif chemokine 28) ELISA Kit measures CCL28 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CCL28 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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