Estradiol ELISA Kit Packaging:96 wells Only those wells that contain

Only those wells that contain Mouse IgG3

When the sample containing Ephrin-A1 is added to the plate

Then a biotinylated detection antibody specific for Human SEPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

cell culture supernates

Estradiol ELISA Kit Packaging:96 wells Only those wells that containThe Estradiol (E2) ELISA kit is based on the principle of delayed competitive binding assay between E2 in the test specimen and E2 enzyme conjugate for a constant amount of anti Estradiol monoclonal antibody epitope (Biotin reagent). In the incubation, anti E2 antibody biotin reagent, E2 standards, controls, and samples are incubated for 45 minutes at room temperature (RT), then E2 enzyme conjugate is added on the top of the reaction mixture and