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Human αMSH in the sample or standard competes with a fixed amount of Human αMSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human αMSH
Then Streptavidin-HRP is added and binds to the Biotinylated ACTC1 antibody
Substrate solution is then added and color develops in proportion to the amount of Human IL1RL2
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL24
Rat MFGE8 (Lactadherin) ELISA Kit Packaging:96 wells Human αMSH in the sampleThe Rat (MFGE8) Lactadherin ELISA Kit measures Lactadherin in Rat samples. The plate has been pre coated with Rat MFGE8 antibody. MFGE8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MFGE8 Antibody is added and binds to MFGE8 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MFGE8 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
