Human IL-4 (Interleukin 4) ELISA Kit 92 KDa Gelatinase Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E2

The concentration of Rat CD8 in the samples is then determined by comparing the OD of the samples to the standard curve

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken IL5

Substrate solution is then added and color develops in proportion to the amount of Mouse PLA2G1B

Human IL-4 (Interleukin 4) ELISA Kit 92 KDa Gelatinase Standards or samples are addedThe Human (IL 4) Interleukin 4 ELISA Kit measures Interleukin 4 in samples. The plate has been pre coated with Human IL4 antibody. IL4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL4 Antibody is added and binds to IL4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.