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The OD value is proportional to the concentration of Human MPIF2
Substrate solution is then added and color develops in proportion to the amount of Rat SOD2
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NPY1R
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Rat SIRT2 (Sirtuin 2) ELISA Kit LECT The OD value is proportionalThe Rat (SIRT2) Sirtuin 2 ELISA Kit measures Sirtuin 2 in Rat samples. The plate has been pre coated with Rat SIRT2 antibody. SIRT2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SIRT2 Antibody is added and binds to SIRT2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SIRT2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
