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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human LTF are added to the micro ELISA plate wells
Then Streptavidin-HRP is added and binds to the Biotinylated ITIH4 antibody
And then biotinylated Human CLEC4C Antibody is added and binds to CLEC4C in the sample
Then a biotinylated detection antibody specific for Mouse LTF/LF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human AMSH (alpha-MSH) ELISA Kit APO2L Samples (or Standards) and HorseradishThe Human AMSH (alpha MSH) ELISA Kit measures AMSH in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with AMSH. AMSH in the sample or standard competes with a fixed amount of biotin labeled AMSH for sites on a pre coated antibody specific to AMSH. Free components are washed off. After adding HRP
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