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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TGFb3
Serpina3N present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Human SLC22A12 Antibody is added and binds to SLC22A12 in the sample
The concentration of Human Irisin in the samples is then determined by comparing the OD of the samples to the standard curve
Human CA14 (Carbonic anhydrase 14) ELISA Kit LVNC6 Standards or samples are addedThe Human CA14 (Carbonic anhydrase 14) ELISA Kit measures CA14 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CA14 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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